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ASU Peptide Array Core Facility

 

Location:

Biodesign Institute
Center for Innovations in Medicine
1001 S. McAllister Ave.
Tempe, AZ 85287-5201
Google Maps

 

Website:

http://www.peptidearraycore.com/

 

Point-of-Contact:

Dr. Doug Daniel
(480) 965-4608
ddaniel@asu.edu

 

Dr. Zhan-Gong Zhao
(480) 965-2330
zhao@asu.edu

About

The ASU Peptide Array Core Facility manufactures and processes peptide microarrays for immunosignaturing and other applications. Immunosignaturing assays may be used to characterize vaccines, determine disease states, and predict disease for diagnosis or prognosis.  In addition to sera or antibodies, other ligands can be bound to the peptide microarrays including proteins, viruses, bacteria, and even eukaryotic cells such as T- or B-cells.

 

Peptide Array Features

  • 125k random peptide sequences per peptide
  • Array size: 0.7cm × 0.7cm
  • 14 µm feature size with 15 µm center to center spacing
  • Synthesized on 8 inch diameter silicon substrate.
  • Can be diced into standard 1 × 3 inch slides.
  • 13 slides/wafer
  • 24 arrays/slide.
  • Typical peptide length is 11 AA

Available Resources

In addition to peptide array synthesis, the PACF offers a number of diagnostic services.  Most of these services are follow-on validation methods to experiments that clients perform on the peptide microarrays.  The core can identify antibody epitopes (bioinformatics methods to identify the linear sequences of the antibody target), serology testing (ELISA or pulldowns using peptide mimotope or epitopes), biochemistry (peptide ligands in affinity columns for antibody enrichment or purification) and bioinformatics analysis of array data. Immunosignaturing assays may be used to characterize vaccines, determine disease states, and predict disease for diagnosis or prognosis.  In addition to sera or antibodies, other ligands can be bound to the peptide microarrays including proteins, viruses, bacteria, and even eukaryotic cells such as T- or B-cells.

News

A new Model P8000 linear track from C&D Semi was installed in April 2016. This equipment allows wafers to be processed in a more automated fashion.